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Publication
Journal of Molecular Biology
Paper
DNA synthesis in vivo in Bacillus subtilis
Abstract
DNA polymerase III is the enzyme responsible for deoxynucleotide addition to nascent DNA fragments in Bacillus subtilis protoplasts. Nascent single-stranded fragments separated from bulk DNA by hydroxyapatite chromatography cannot self-anneal. Partial inhibition of DNA polymerase III by 6-(hydroxyphenylazo)-uracil, a specific inhibitor, slows the rate of nascent fragment synthesis but has no effect on final size. Neither DNA polymerase I nor II can elongate nascent fragments in protoplasts when DNA polymerase III is completely inhibited. © 1976.