The first decade of genome sequencing saw a surge in the characterization of proteins with unknown functionality. Even still, more than 20% of proteins in well-studied model animals have yet to be identified, making the discovery of their active site one of biology's greatest puzzle. Herein, we apply a Transformer architecture to a language representation of bio-catalyzed chemical reactions to learn the signal at the base of the substrate-active site atomic interactions. The language representation comprises a reaction simplified molecular-input line-entry system (SMILES) for substrate and products, complemented with amino acid (AA) sequence information for the enzyme. We demonstrate that by creating a custom tokenizer and a score based on attention values, we can capture the substrate-active site interaction signal and utilize it to determine the active site position in unknown protein sequences, unraveling complicated 3D interactions using just 1D representations. This approach exhibits remarkable results and can recover, with no supervision, 31.51% of the active site when considering co-crystallized substrate-enzyme structures as a ground-truth, vastly outperforming approaches based on sequence similarities only. Our findings are further corroborated by docking simulations on the 3D structure of few enzymes. This work confirms the unprecedented impact of natural language processing and more specifically of the Transformer architecture on domain-specific languages, paving the way to effective solutions for protein functional characterization and bio-catalysis engineering.