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Publication
Pure and Applied Chemistry
Paper
The Kinetic Parameters of Carbonic Anhydrase by 13C Nmr
Abstract
Carbonic anhydrase, an enzyme found in red blood cells, catalyses the reversible hydration of carbon dioxide: CO2+ H2O ⇄ H++ HCO-3. We have used measurements of the 13C n.m.r. linewidths of 13CO2and H13CO─3in solutions of the native human B enzyme to obtain the kinetic parameters that describe the enzymatic activity. The measurements, made under thermal equilibrium conditions and with no added buffer, were taken as a function of pH and substrate concentration at 25°C, and at pH 7.7 over a range of temperatures. The kinetic results, which compare well with published results obtained by stopped-flow methods in buffered solutions, are discussed in terms of the several models that have been suggested to describe the enzymatic mechanism. © 1974, Walter de Gruyter. All rights reserved.