In this paper a brief overview of the third workshop on Multimodal Analyses enabling Artificial Agents in Human- Machine Interaction. The paper is focussing on the main aspects intended to be discussed in the workshop reflecting the main scope of the papers presented during the meeting. The MA3HMI 2016 workshop is held in conjunction with the 18th ACM International Conference on Mulitmodal Interaction (ICMI 2016) taking place in Tokyo, Japan, in November 2016. This year, we have solicited papers concerning the different phases of the development of multimodal systems. Tools and systems that address real-Time conversations with artificial agents and technical systems are also within the scope.